Welcome leaders of Jilin Provincial Department of Commerce to Qijian Bio-Pharmaceutical for investigation
2024-04-02
Sequences of primers commonly used in RT-PCR
- Categories:Industry news
- Author:
- Origin:
- Time of issue:2018-09-28
- Views:361
(Summary description)
Primers should be diluted with TE. Oligo is unstable under acidic conditions and is easily degraded. If the primers are dissolved in water, the pH value cannot be guaranteed. If the synthetic primers are not well purified and cause partial acidity, the primers will degrade over time and cannot be used. Moreover, high-purity water itself is acidic, which is not conducive to storage. Therefore, the standard operation should be followed, and the primers should be dissolved in 1XTE, so that the Buffer can ensure that the pH is 7.5-8, and the primers can be stored. If you want to store for a longer time, it is better to use dry powder.
RT-PCR primer sequences
Gene source Primer sequence Product size (kb)
β-actin Human Sense chain CCTCG CCTTT GCCGA TCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62 kb
β-actin* Rat Sense chain TACAA CCTCC TTGCA GCTCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62kb
β-actin mouse Sense chain GTCGT ACCAC AGGCA TTGTG ATGG Antisense chain GCAAT GCCTG GGTAC ATGGT GG 0.49 kb
GAPDH human sense chain GGTGA AGGTC GGAGT CAACG antisense chain CAAAG TTGTC ATGGA TGHACC 0.50kb
GAPDH rat Sense chain GATGC TGGTG CTGAG TATGR CG antisense chain GTGGT GCAGG ATGCA TTGCT CTGA 0.20 kb
Dynein mouse sense chain GCGGG CGCTG GAGGA GAA antisense chain GGATC TTCAT GAGGT AGTCA GTC 12.3 kb
Polymerase ε Human Sense chain CGCCA AATTT CTCCC CTGAAA Antisense chain CCGTA GTGCT GGGCA ATGTT C 6.8 kb
Polymerase ε Human Sense chain AAGGC TGGCG GATTA CTGCC Antisense chain GATGC TGCTG GTGAT GTACT C 3.5 kb
Tuberous Sclerosis human sense chain GGAGT TTATC ATCAC CGCGG AAATA CTGAG AG antisense chain TATTT CACTG ACAGG CAATA CCGTC CAAGG 5.3 kb
18S rRNA soybean Sense strand CTTTC GATGG TAGGA TAGTG GCCT Antisense strand CAATG ATCCT TCCGC AGGTT CACCT AC 1.5 kb
*Primers will not amplify pseudogenes
PCR primer sequence Gene source Primer sequence Product size (kb)
HIV gag region virus SK 38ATTAAT CACTA TCCAG TAGGA GAAAT
SK 39TTTGG TCCTG TCTTA TGTCC AGAAT GC 0.11kb
β-globin human (29923) GGTGT TCCCT TGATG TAGCA CA
(34016) CCAGG ATTTT TGATG GGACA CG 4.1kb
β-globin human (31194) GCTGC TCTGT GCATC CGAGT GG
(34016) CCAGG ATTTT TGATG GGACA CG 2.8kb
Sequence source: nvitrogen company
Sequences of primers commonly used in RT-PCR
(Summary description)
Primers should be diluted with TE. Oligo is unstable under acidic conditions and is easily degraded. If the primers are dissolved in water, the pH value cannot be guaranteed. If the synthetic primers are not well purified and cause partial acidity, the primers will degrade over time and cannot be used. Moreover, high-purity water itself is acidic, which is not conducive to storage. Therefore, the standard operation should be followed, and the primers should be dissolved in 1XTE, so that the Buffer can ensure that the pH is 7.5-8, and the primers can be stored. If you want to store for a longer time, it is better to use dry powder.
RT-PCR primer sequences
Gene source Primer sequence Product size (kb)
β-actin Human Sense chain CCTCG CCTTT GCCGA TCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62 kb
β-actin* Rat Sense chain TACAA CCTCC TTGCA GCTCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62kb
β-actin mouse Sense chain GTCGT ACCAC AGGCA TTGTG ATGG Antisense chain GCAAT GCCTG GGTAC ATGGT GG 0.49 kb
GAPDH human sense chain GGTGA AGGTC GGAGT CAACG antisense chain CAAAG TTGTC ATGGA TGHACC 0.50kb
GAPDH rat Sense chain GATGC TGGTG CTGAG TATGR CG antisense chain GTGGT GCAGG ATGCA TTGCT CTGA 0.20 kb
Dynein mouse sense chain GCGGG CGCTG GAGGA GAA antisense chain GGATC TTCAT GAGGT AGTCA GTC 12.3 kb
Polymerase ε Human Sense chain CGCCA AATTT CTCCC CTGAAA Antisense chain CCGTA GTGCT GGGCA ATGTT C 6.8 kb
Polymerase ε Human Sense chain AAGGC TGGCG GATTA CTGCC Antisense chain GATGC TGCTG GTGAT GTACT C 3.5 kb
Tuberous Sclerosis human sense chain GGAGT TTATC ATCAC CGCGG AAATA CTGAG AG antisense chain TATTT CACTG ACAGG CAATA CCGTC CAAGG 5.3 kb
18S rRNA soybean Sense strand CTTTC GATGG TAGGA TAGTG GCCT Antisense strand CAATG ATCCT TCCGC AGGTT CACCT AC 1.5 kb
*Primers will not amplify pseudogenes
PCR primer sequence Gene source Primer sequence Product size (kb)
HIV gag region virus SK 38ATTAAT CACTA TCCAG TAGGA GAAAT
SK 39TTTGG TCCTG TCTTA TGTCC AGAAT GC 0.11kb
β-globin human (29923) GGTGT TCCCT TGATG TAGCA CA
(34016) CCAGG ATTTT TGATG GGACA CG 4.1kb
β-globin human (31194) GCTGC TCTGT GCATC CGAGT GG
(34016) CCAGG ATTTT TGATG GGACA CG 2.8kb
Sequence source: nvitrogen company
- Categories:Industry news
- Author:
- Origin:
- Time of issue:2018-09-28
- Views:361
Information
Primers should be diluted with TE. Oligo is unstable under acidic conditions and is easily degraded. If the primers are dissolved in water, the pH value cannot be guaranteed. If the synthetic primers are not well purified and cause partial acidity, the primers will degrade over time and cannot be used. Moreover, high-purity water itself is acidic, which is not conducive to storage. Therefore, the standard operation should be followed, and the primers should be dissolved in 1XTE, so that the Buffer can ensure that the pH is 7.5-8, and the primers can be stored. If you want to store for a longer time, it is better to use dry powder.
RT-PCR primer sequences
Gene source Primer sequence Product size (kb)
β-actin Human Sense chain CCTCG CCTTT GCCGA TCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62 kb
β-actin* Rat Sense chain TACAA CCTCC TTGCA GCTCC Antisense chain GGATC TTCAT GAGGT AGTCA GTC 0.62kb
β-actin mouse Sense chain GTCGT ACCAC AGGCA TTGTG ATGG Antisense chain GCAAT GCCTG GGTAC ATGGT GG 0.49 kb
GAPDH human sense chain GGTGA AGGTC GGAGT CAACG antisense chain CAAAG TTGTC ATGGA TGHACC 0.50kb
GAPDH rat Sense chain GATGC TGGTG CTGAG TATGR CG antisense chain GTGGT GCAGG ATGCA TTGCT CTGA 0.20 kb
Dynein mouse sense chain GCGGG CGCTG GAGGA GAA antisense chain GGATC TTCAT GAGGT AGTCA GTC 12.3 kb
Polymerase ε Human Sense chain CGCCA AATTT CTCCC CTGAAA Antisense chain CCGTA GTGCT GGGCA ATGTT C 6.8 kb
Polymerase ε Human Sense chain AAGGC TGGCG GATTA CTGCC Antisense chain GATGC TGCTG GTGAT GTACT C 3.5 kb
Tuberous Sclerosis human sense chain GGAGT TTATC ATCAC CGCGG AAATA CTGAG AG antisense chain TATTT CACTG ACAGG CAATA CCGTC CAAGG 5.3 kb
18S rRNA soybean Sense strand CTTTC GATGG TAGGA TAGTG GCCT Antisense strand CAATG ATCCT TCCGC AGGTT CACCT AC 1.5 kb
*Primers will not amplify pseudogenes
PCR primer sequence Gene source Primer sequence Product size (kb)
HIV gag region virus SK 38ATTAAT CACTA TCCAG TAGGA GAAAT
SK 39TTTGG TCCTG TCTTA TGTCC AGAAT GC 0.11kb
β-globin human (29923) GGTGT TCCCT TGATG TAGCA CA
(34016) CCAGG ATTTT TGATG GGACA CG 4.1kb
β-globin human (31194) GCTGC TCTGT GCATC CGAGT GG
(34016) CCAGG ATTTT TGATG GGACA CG 2.8kb
Sequence source: nvitrogen company
Scan the QR code to read on your phone
Previous:
Research is a choice in life
Previous :
Research is a choice in life
Related News
Contact Us
Contact us
客户留言
Description:
Contact Us
ADD： No.707 Chaofan street, High-Tech Industrial Development Zone, Changchun City, Jilin province, China
Service Hotline：0086-431-81285900
Fax：0086-431-88794098
Email：export@qijianbio.com
Copyright：Jilin Qijian Bio-pharmaceutical Co., Ltd. by:300.cn 吉ICP备20003293号 SEO
搜索