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Recently, the large-scale withdrawal of Chinese papers by foreign journals has been raging. The Japanese Institute of Physical C

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2018/09/28 23:21
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Primers should be diluted with TE. Oligo is unstable under acidic conditions and is easily degraded. If the primer is dissolved in water, the pH cannot be guaranteed. If the synthetic primer is not well purified and the acid is too acidic, the primer will degrade after a certain period of time and cannot be used. Moreover, high-purity water itself is acidic and not conducive to storage. Therefore, it should be operated according to the standard, and the primers are dissolved in 1XTE, so that Buffer can be used to ensure pH 7.5-8, and the primer can be stored for a long time. If you want to save for a longer period of time, it is best to dry powder.
 
RT-PCR primer sequence
 
Gene source primer sequence product size (kb)
 
--actin human sense strand CCTCG CCTTT GCCGA TCC antisense strand GGATC TTCAT GAGGT AGTCA GTC 0.62 kb
 
--actin* rat sense chain TACAA CCTCC TTGCA GCTCC antisense strand GGATC TTCAT GAGGT AGTCA GTC 0.62kb
 
--actin mouse sense chain GTCGT ACCAC AGGCA TTGTG ATGG antisense strand GCAAT GCCTG GGTAC ATGGT GG 0.49 kb
 
GAPDH human sense strand GGTGA AGGTC GGAGT CAACG antisense strand CAAAG TTGTC ATGGA TGHACC 0.50kb
 
GAPDH rat sensed chain GATGC TGGTG CTGAG TATGR CG antisense strand GTGGT GCAGG ATGCA TTGCT CTGA 0.20 kb
 
Dynein mouse sense strand GCGGG CGCTG GAGGA GAA antisense strand GGATC TTCAT GAGGT AGTCA GTC 12.3 kb
 
Polymerase ε Human Meaning Chain CGCCA AATTT CTCCC CTGAAA Antisense Chain CCGTA GTGCT GGGCA ATGTT C 6.8 kb
 
Polymerase ε human sense strand AAGGC TGGCG GATTA CTGCC antisense strand GATGC TGCTG GTGAT GTACT C 3.5 kb
 
Tuberous Sclerosis Human Significance Chain GGAGT TTATC ATCAC CGCGG AAATA CTGAG AG Antisense Chain TATTT CACTG ACAGG CAATA CCGTC CAAGG 5.3 kb
 
18S rRNA Soybean sense strand CTTTC GATGG TAGGA TAGTG GCCT antisense strand CAATG ATCCT TCCGC AGGTT CACCT AC 1.5 kb
 
* Primers do not amplify pseudogenes
 
PCR primer sequence gene source primer sequence product size (kb)
 
HIV gag region virus SK 38ATTAAT CACTA TCCAG TAGGA GAAAT
 
SK 39TTTGG TCCTG TCTTA TGTCC AGAAT GC 0.11kb
 
--globin person (29923)GGTGT TCCCT TGATG TAGCA CA
 
(34016) CCAGG ATTTT TGATG GGACA CG 4.1kb
 
--globin person (31194) GCTGC TCTGT GCATC CGAGT GG
 
(34016) CCAGG ATTTT TGATG GGACA CG 2.8kb
 
Sequence source: nvitrogen