Industry news_News_Qijian Bio-Pharmaceutical Co.,Ltd.

Qijian was invited to participate in "China Service Trade Fair 2021”

Our company was invited to participate in the "China Service Trade Fair 2021" which held in Beijing from September 4 to 7, 2021, Li Wei, Vice Governor of Jilin Province, visited Qijian 's booth, he encouraged us to strengthen exchanges and promote the development of service trade.

10 2021/09

Qijian was invited to participate in "China Service Trade Fair 2021”

New recombinant human growth hormone sustained-release microcapsules successfully developed

Recently, the Institute of Process Engineering, Chinese Academy of Sciences learned that a team led by Ma Guanghui, a researcher at the Institute, developed a recombinant human growth hormone sustained-release microcapsule with uniform particle size. The relevant results were published in the international academic journal Molecular Pharmacy. It is understood that recombinant human growth hormone (rhGH) is widely used in clinical treatment of short stature, severe burns, lipodystrophy in AIDS patients and other diseases. However, the half-life of rhGH is short, and frequent injections are required to achieve effective plasma concentrations, resulting in poor patient compliance. In response to this problem, scientists have conducted a lot of research on rhGH sustained-release microcapsules, but there are still common problems such as uneven particle size of microcapsules, unsustainable release, and poor drug stability. At the same time, the acidic products produced during the degradation of traditional hydrophobic material microcapsules can cause inflammation at the injection site and cause serious side effects. Therefore, the development of safe and effective rhGH sustained-release microcapsules has important research significance. This time, Ma Guanghui's team first used the W/O/W double emulsion method to load rhGH into the amphiphilic polylactic acid-polyethylene glycol copolymer emulsion, and combined with the rapid membrane emulsification technology to achieve the uniformity of the particle size of the microspheres . Subsequent in vivo rat model experiments show that the preparation can effectively prolong the release of rhGH in the body, and maintain the activity of rhGH well, and the rat bones grow significantly, which improves the therapeutic effect. In addition, compared with traditional polylactic acid (PLA) and polylactic acid-co-glycolic acid (PLGA) microcapsules, the preparation does not produce inflammatory reactions and has no effect on the functions of major organs such as the heart, liver, and kidneys. Safe sustained release carrier. The research work was supported by the "973" project and the National Natural Science Foundation of China.

28 2018/09

New recombinant human growth hormone sustained-release microcapsules successfully developed

Cell: Breakthrough! Scientists identify key gene that is expected to promote cardiomyocyte regeneration to form heart tissue!

March 6, 2018 /Bio Valley BIOON/ -- Recently, in a study published in the international journal Cell, scientists from the Gladstone Institutes (Gladstone Institutes) A key gene for adult cell division and proliferation; some organisms have the ability to regenerate tissue remarkably. If fish and salamanders suffer heart damage, their cells will continue to divide and successfully repair damaged organs. Imagine if we also What would it be like to have this regenerative ability? In the embryo, human heart cells can sort and proliferate to promote heart growth and development; but the problem is that right after birth, the body's cardiomyocytes lose the ability to divide, unlike many cells in other tissues of the body. The same, including the brain, spinal cord, and pancreas. Dr Deepak Srivastava explained that because many adult cells cannot divide, the body cannot replace the lost cells, which can lead to disease. If we can find a new way to make these cells divide again, then we may hope Achieving the regenerative capacity of a range of tissues. Scientists have been trying to do this for decades, with little success; so far, these attempts have been ineffective and difficult to replicate; in this latest study, researchers may hope To achieve this ultimate goal, the researchers developed the first efficient and stable method to create adult cardiomyocytes that can divide and repair damaged heart tissue during a heart attack (at least in animal models). Of the more than 24 million patients with heart failure worldwide, there are few treatment options for patients other than heart transplants to treat patients with advanced disease; instead, cells divide to make new cardiomyocytes (similar to (like salamanders) may be able to help millions of patients repair damaged heart tissue. Unlock the potential of adult cell reproduction In this study, researchers Srivastava and colleagues identified four genes that control the cell division cycle. They found that when combined, these genes promote mature cardiomyocytes to re-enter the cell cycle, thereby causing Promotes cell division and rapid proliferation. "When the function of all four genes is increased simultaneously, adult cells begin to divide again and regenerate heart tissue," said researcher Tamer Mohamed. "We also found that when patients developed heart failure, combining these genes significantly improved the hearts of patients. Function. The researchers then tested the new technique in animal models and in cardiomyocytes derived from human stem cells, using a rigorous technique to track whether these adult cells could actually make dividing cells that carry special colors that can be easily monitored. To divide in the heart, the results showed that in the mixed mode of the four genes, 15%-20% of the cardiomyocytes were able to divide and remain active. Compared to previous findings (1%), this new technology may increase the efficiency and reliability of cell division. Of course, in human organs, gene application should be strictly monitored, because excessive or redundant cells Division often induces tumors; researchers will simplify the technique in later studies by finding ways to reduce the number of genes needed for cell division (while still maintaining the efficiency of cell division), and it turns out that using Two drug-like molecules to remove two of the genes yielded the same findings. Regenerates a variety of human tissues The researchers believe that this new technology may be used to induce other types of adult cells to divide again, and the four genes they used in the article are not unique to heart tissue; in the end, Srivastava said, heart cells are important to us. It's especially challenging because cardiomyocytes exit the cell cycle after an individual is born, and the cell's state is locked, which may explain why people don't develop heart tumors; The new technology for re-dividing these "difficult" cells may also be used in the future to "unlock" the potential of other types of cells to divide, including nerve cells, pancreatic cells, hair cells in the ear and retinal cells. Based on the results of this paper, the researchers hope that through more in-depth research in the future, more powerful regenerative technologies can be developed to treat not only heart failure, but also many types of human diseases, including brain damage, diabetes, hearing loss and Blindness, etc.; researchers believe that the ability of human tissue to regenerate will one day surpass that of salamanders. (Qjbio qjbio.com.cn)

28 2018/09

Cell: Breakthrough! Scientists identify key gene that is expected to promote cardiomyocyte regeneration to form heart tissue!

List of recent progress in bioengineering research

1. Nat Commun: Bioengineering miniature silkworm cocoons to protect drug molecules has miraculous effects! Scientists have created a tiny silkworm cocoon that protects sensitive drug molecules from degradation during storage and release. They hope the technology will extend the shelf life of drugs and help treat cancer, neurodegenerative diseases and more. For scientists, finding inspiration from nature to facilitate human life is a common method. In the past, scientists have been inspired by enzymatic reactions in nature to produce energy and by cacti to store water. Today, we use the natural properties of silkworm pupae to create carriers suitable for small molecules. "Many drugs have excellent therapeutic effects, but have low stability and are difficult to store. This is a common problem in medical practice," said the study's lead author, Tuomas Knowles from the University of Cambridge. Therefore, they hoped that the cocoons could help prolong the lifespan of the drug molecules. Because silk is a biodegradable remnant and is inexpensive to produce, it has been used to make other surgical supplies, including brain implants, optical devices, Xibao Home, and sticky glue, to name a few. The safety of the modified material has been proven. In order to transform silkworm cocoons for human use, the researchers used a unique micro-engineering technology to produce a miniature simulated silkworm cocoon, which is one-thousandth the volume of a natural silkworm cocoon. Afterwards, the researchers tested the protective effect of the miniature silkworm cocoons on antibodies. Antibodies are known to be the most difficult type of drug to preserve. At higher concentrations, antibodies tend to aggregate and it is difficult to maintain the original stability. "By wrapping the antibody inside a tiny silkworm cocoon, we were able to significantly enhance its lifespan and also expand the range of uses for antibody drugs," says Knowles. "We are very pleased that the engineered micromaterials have new uses." The results were published in the journal Nature Communications. 2. Sci Trans Med: Successful expansion of engineered liver tissue after transplantation Many diseases, including cirrhosis and hepatitis, can lead to liver failure. There are currently more than 17,000 patients in the United States suffering from liver failure and anxiously awaiting liver transplants. However, available liver donors are in short supply. To address the shortage of donors, the researchers successfully developed engineered liver tissue. That is, by encapsulating three different types of cells in a biodegradable tissue framework. In a mouse model of liver injury disease, the researchers found that the engineered liver expanded about 50-fold after being transplanted into the mouse abdominal cavity, and was eventually able to perform full liver function. This engineered liver could help millions of patients suffering from chronic liver disease who do not have a suitable donor. The results were published in the latest issue of the journal Science Translational Medicine. "These patients do not need a donor transplant urgently, but they do have liver disease, and if the engineered liver can end up in the clinic, it will help these patients at the same time," said Dr. Kelly Stevens, an author on the paper. In 2011, Bhatia et al. developed an engineered tissue framework that can be transplanted into the abdominal cavity of mice. After that, the liver cells in the frame can integrate with the original circulatory system of the mouse, so as to obtain blood supply and realize the function of the liver. However, this structure can contain fewer than 1 million liver cells, whereas a healthy human liver has at least 100 billion cells. Bhatia believes that to help liver patients, at least 10% to 30% of the liver cells need to be transplanted. To increase the number of liver cells, the researchers hoped that the initially transplanted liver cells would replicate themselves. "The liver is the only mature organ in our body that can proliferate," Bhatia said. In collaboration with other researchers, Bhatia et al. placed hepatocytes, fibroblasts, and endothelial cells into miniature liver structures, and eventually allowed them to proliferate and differentiate in vivo to form a replacement liver. When the engineered liver was transplanted into mice, it was able to be influenced by signals from the peripheral environment. These signals, including growth factors, enzymes, and other molecules, are naturally produced when the liver is damaged, and can activate endothelial cells to form blood vessels and activate liver cells to proliferate to a nearly 50-fold expansion into normal liver tissue. The researchers believe that if the technology can enter clinical practice, it will offer new hope for the majority of liver patients waiting for transplants. 3. Nature: Heavy! Bioengineered human liver tissue developed to mimic natural development I

28 2018/09

List of recent progress in bioengineering research

Top 10 must-see heavyweight research in May

[1] Mol Ther: Heavy! Scientists successfully use CRISPR/Cas9 to eliminate HIV-1 infection in live animals doi: 10.1016/j.ymthe.2017.03.012 Because the virus can hide in the underlying virus reservoir, the hope of a complete cure for HIV infection is still very slim. Recently, in a study published in the international journal Molecular Therapy, researchers from Temple University and the University of Pittsburgh The combined study found that they were able to remove HIV DNA from the genome of living animals to eliminate late HIV infection, and the researchers achieved this "feat" for the first time in three different animal models, including human Induced mouse model (transplanted into human immune cells) and virus-infected mouse model, etc. In the article, the researchers found for the first time that the use of "gene magic scissors" CRISPR/Cas9 can completely shut down the replication of HIV-1 and eliminate the virus in infected cells of animals. The study builds on a 2016 proof-of-concept study conducted by researchers using genetically modified rat and mouse models in which HIV-1 DNA was incorporated into the genome of every tissue in the animal model; It was found that this strategy was able to remove HIV-1-targeted segments from the genomes of most tissues in the experimental animals. [2] Genes Dev: Scientists find potential cells and genes that lead to less whiteheads and baldness doi: 10.1101/gad.298703.117 Researchers from Southwestern Medical Center have recently discovered cells that directly form hair and uncovered the mechanism that causes graying of hair. These findings may help find a treatment for baldness and graying of hair. "This project started out to study the process of tumor formation, but we ended up discovering why hair turns gray and identifying cells that directly form hair. With this knowledge, we hope to develop a therapeutic drug in the future. Or a safe treatment to deliver the genes necessary for the hair follicle to the cells to correct the errors that occur in the cells," said Professor Lu Le, author of the paper. The researchers found that a protein called KROX20 turns on expression in skin cells that can form hair shafts. These progenitor cells produce another protein called stem cell factor (SCF), which is important for hair color. When the researchers deleted the SCF gene in the progenitor cells of a mouse model, the mice's hair turned white. If the KROX20-expressing cells were removed, hair would not grow, and the mice developed alopecia areata. 　　 [3] Nat Med: Human rejuvenation is expected to be realized! Scientists have found that marijuana may reverse human brain aging doi: 10.1038/nm.4311 Memory declines with age; in a recent study published in Nature Medicine, researchers from the University of Bonn and the Hebrew University of Jerusalem found through a joint study that marijuana can reverse the aging process of the brain , researchers found that after long-term low-dose treatment with cannabis active ingredients, the state of old mice can return to the state of being born at two months, which may be for later researchers to develop new treatments for brain diseases such as dementia Offers new clues and hope. Like other organs in the body, our brains also age, and ultimately our cognitive abilities decline with age, and it's worth noting that as we age, it's harder to remember new things at the same time Or focusing on multiple things at once, a normal process that can lead to dementia, so researchers have been trying to find new ways to slow or reverse the aging effects of aging on the brain. Now researchers have carried out related studies on mice, which have a short lifespan in the natural state and tend to show significant cognitive impairment when they are 12 months old; in this study, the researchers put a small amount of The active ingredient of cannabis, THC, was injected into 2-month-old, 12-month-old and 18-month-old mice (for a period of 4 weeks). The researchers then examined the learning and memory performance of these mice. They found that mice given a placebo alone exhibited natural age-dependent learning and memory deficits, compared to those given THC, the active ingredient in cannabis. The cognitive function of the mice was as good as that of the 2-month-old control mice, and the researchers believe that the treatment may be able to completely reverse the aging animals' memory loss and other symptoms. 【4】Nature: Surprising! A common cerebrovascular disease is actually associated with the gut microbiome doi: 10.1038/nature22075 According to a new study, researchers from the Perelman School of Medicine at the University of Pennsylvania report that bacteria in the gut microbiome promote the formation of intracranial cavernous malformation (CCM). This study suggests that altering the gut microbiome of CCM patients may be an effective treatment for this cerebrovascular disease. The related research results were published onlin

28 2018/09

Top 10 must-see heavyweight research in May

Bacteria's integrated protein assembly line revealed for the first time

Many processes that take place in cells are integral to life. As two of them, transcription and translation allow the genetic information stored in DNA to be decoded into the proteins that form the proteins of all organisms such as bacteria, plants and humans. Scientists have known for half a century that the two processes are coupled in bacteria, but until now, they didn't know how. Now, in a new study, researchers from the University of Wisconsin-Madison (UW-Madison) and the Max Planck Institute for Biophysical Chemistry in Germany have revealed that a so-called "expressome" the exact structure of the complex. The results of the study were published in the April 14, 2017 issue of Science, with the title "Architecture of a transcribing-translating expressome". The corresponding authors of the paper are Robert Landick, a professor in the UW-Madison Department of Biochemistry, and Patrick Cramer, director of the Max Planck Institute for Biophysical Chemistry. The researchers say the study using the model organism Escherichia coli could help to understand how the bacteria affect human health, including better understanding gene regulation and developing new antibiotics. Landick explained, "The existence of this complex in bacteria has been proposed based on evidence, but so far, no one has confirmed its existence. This study is the first to demonstrate the ability to harness these two already complex machines. (i.e. transcription complex, or transcription machinery; translation complex, or translation machinery) forms a larger supercellular machinery (i.e. expressosome)." The transcription process uses RNA polymerase to convert DNA into RNA. Following this process, another molecular machine called the ribosome translates this RNA (more specifically, signaling RNA, or mRNA) into a protein that the bacteria can use to function. Rachel Mooney, a researcher in UW-Madison's Department of Biochemistry, said that in bacterial expressosomes, RNA polymerase and ribosomes form a complex structure to carry out the two processes in a coupled fashion, and the newly resolved The expression body structure helps to understand how this happens. Transcription and translation also occur in animals and humans, but the two processes are not coupled as in bacteria. Instead, they occur in two physically distinct parts of the cell. If scientists can discover a way to disrupt this expressosome, they may be able to develop drugs that target bacteria but do not harm human cells, the researchers said. These findings also extend to the study of the microbiome (the population of microbes in and on the human body). Ongoing research demonstrates how important the microbiome is to human health, and understanding gene regulation in these microbial populations is a vital part of these efforts. Today, this expressosome structure is the basis for this understanding. "In human biology, we tend to think of what happens in human cells, but there are at least as many bacterial cells inside and on our bodies as there are human cells," Landick said. is not common, then we used it as a model organism to extend our research to other bacteria that are critical to human processes." Landick, Mooney (UW-Madison team) collaborated with Cramer and Rebecca Kohler (German team) on the study. Equipment provided by the German team assisted in deciphering the structure of the expressosome. This expression body contains RNA polymerase provided by the UW-Madison team. "Our study explains past observations that these two processes (transcription and translation) are coupled together in these bacterial cells," Cramer said. Researchers are also interested in the origin of this complex. Why these two processes are coupled in bacteria but not in organisms such as humans needs to be studied from an evolutionary perspective. Landick explained, "One of the arguments against it is to simply think that bacteria are far ahead of us in evolution. It's counterintuitive, but only technically, they have far more descendants than us. Bacteria The evolutionary pressures faced have led to this very integrated and very efficient approach to transcribing and translating DNA into protein."

28 2018/09

Bacteria's integrated protein assembly line revealed for the first time